Qualitative reproduction of experimental fluxes from published work (Krämer and Klingenberg, 1982). (A and B) ATP uptake rate vs. external ATP concentration for a membrane potential (ΔΨ) of 0 and 180 mV in yellow and green, respectively. Black and grey points describe the ADP uptake rate vs. external ADP concentration for a membrane potential of 0 and 180 mV. (A and B) Transformed data from Krämer and Klingenberg (1982) (A; we followed the same approach as in Metelkin et al. [2006]), and our simulations (B), the flux was computed calculating an average over 30 s. ANP stands for ATP for the yellow and green points, and for ADP for black and grey points. (C and D) ADP uptake rate vs. external ADP concentration for membrane potential (ΔΨ) of 180 mV and ATP concentrations outside the liposomes (To) of 0, 100, 400 µM shown in black, light grey, and yellow points, respectively. Green, grey, and red points describe the dependency with the membrane potential of 0 mV and concentrations of ATP concentrations outside the liposomes (To) of 0, 20, 100 µM, respectively. The concentrations of ADP and ATP inside the liposomes were 5 mM. (E) ATP uptake rate vs. ATP concentration, yellow and green points for a membrane potential (ΔΨ) of 0 and 180 mV, respectively, and ADP concentration equal to the ATP concentration. (F) ADP uptake rate vs. ADP concentration, black and grey points for a membrane potential of 0 and 180 mV, respectively, and ATP concentration equal to the ADP concentration.
Figure S1.

Qualitative reproduction of experimental fluxes from published work ( Krämer and Klingenberg, 1982 ). (A and B) ATP uptake rate vs. external ATP concentration for a membrane potential (ΔΨ) of 0 and 180 mV in yellow and green, respectively. Black and grey points describe the ADP uptake rate vs. external ADP concentration for a membrane potential of 0 and 180 mV. (A and B) Transformed data from Krämer and Klingenberg (1982) (A; we followed the same approach as in Metelkin et al. [2006]), and our simulations (B), the flux was computed calculating an average over 30 s. ANP stands for ATP for the yellow and green points, and for ADP for black and grey points. (C and D) ADP uptake rate vs. external ADP concentration for membrane potential (ΔΨ) of 180 mV and ATP concentrations outside the liposomes (To) of 0, 100, 400 µM shown in black, light grey, and yellow points, respectively. Green, grey, and red points describe the dependency with the membrane potential of 0 mV and concentrations of ATP concentrations outside the liposomes (To) of 0, 20, 100 µM, respectively. The concentrations of ADP and ATP inside the liposomes were 5 mM. (E) ATP uptake rate vs. ATP concentration, yellow and green points for a membrane potential (ΔΨ) of 0 and 180 mV, respectively, and ADP concentration equal to the ATP concentration. (F) ADP uptake rate vs. ADP concentration, black and grey points for a membrane potential of 0 and 180 mV, respectively, and ATP concentration equal to the ADP concentration.

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