DCAF13 is required for peripheral T cell survival. (A) Flow cytometry analysis of the percentages of CD4⁺ and CD8⁺ T cell subsets in lymph nodes and spleens from CD4 Cre⁻Dcaf13^fl/fl (WT) and CD4 Cre⁺Dcaf13^fl/fl (cKO) mice (left) and the corresponding percentage quantitation (right) (n = 5) are shown. (B) Quantitation of the cell numbers of CD4⁺ and CD8⁺ T cell subsets in lymph nodes and spleens, as total splenocytes or lymphocytes multiplied by the percentage of the corresponding subset found in the total population (n = 5). (C) Flow cytometry analysis of bone marrow transplantation reconstituted in CD45.1⁺-irradiated B6 wild-type mice. The bone marrow cells injected into the host mice were composed of CD45.1⁺ B6 (WT) bone marrow cells mixed with CD45.2⁺ CD4 Cre⁻Dcaf13^fl/fl (WT) or CD4 Cre⁺Dcaf13^fl/fl (cKO) bone marrow cells. CD45.1⁺ cells served as internal controls. The data shown are the percentages of CD45.2⁺ (DCAF13 cKO or WT) subsets gated on CD4⁺ or CD8⁺ T cell populations in lymph nodes and spleens from the host mice after 8 wk. Representative flow cytometry pictures (left) and corresponding quantitation (right; WT, n = 4; cKO, n = 6) are shown. Data are presented as the mean ± SD. A two-tailed Student’s t test was used. ns is not significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.