Figure S3.
Optical measurement of Na concentration changes during and after Na influx activated by veratridine (3 μM). Myocytes were loaded with CoronaGreen AM Na-selective dye (5 μM AM) for 30 min at 25°C (Iamshanova et al., 2016). Myocytes were patch-clamped using Na-free NMG solutions as in Fig. 8. Na was applied and removed multiple times without veratridine and subsequently with veratridine. In the presence of extracellular Na (120 mM), cytoplasmic CoronaGreen fluorescence increases by ∼5% and decreases again upon removal of extracellular Na. Fluorescence changes increase by about a factor of two in the presence of veratridine. Each fluorescence response was fit to falling or rising exponential functions (red curves), and the average time constant amounted to 28 s. The magnitudes of fluorescence changes (5–10%) are consistent with Na changes of 2–4 mM, assuming that CoronaGreen has a dissociation constant (Kd) of 10 mM for Na.

Optical measurement of Na concentration changes during and after Na influx activated by veratridine (3 μM). Myocytes were loaded with CoronaGreen AM Na-selective dye (5 μM AM) for 30 min at 25°C (Iamshanova et al., 2016). Myocytes were patch-clamped using Na-free NMG solutions as in Fig. 8. Na was applied and removed multiple times without veratridine and subsequently with veratridine. In the presence of extracellular Na (120 mM), cytoplasmic CoronaGreen fluorescence increases by ∼5% and decreases again upon removal of extracellular Na. Fluorescence changes increase by about a factor of two in the presence of veratridine. Each fluorescence response was fit to falling or rising exponential functions (red curves), and the average time constant amounted to 28 s. The magnitudes of fluorescence changes (5–10%) are consistent with Na changes of 2–4 mM, assuming that CoronaGreen has a dissociation constant (Kd) of 10 mM for Na.

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