Figure S2.
Diffusion of four additional fluorescent molecules into the cytoplasm of patch-clamped murine myocytes. Same experimental conditions as Figs. 6 and 7. Dashed lines indicate the positions of the line scans shown at right. Yellow dots indicate the end of the myocyte in proximity to the pipette tip. (A) 2 kD FITC-labeled PEG with one amino group (100 μM). (B) 2 kD FITC-labelled PEG with one carboxyl group (100 μM). (C) 4.4 kD TRITC-labeled dextran (10 μM). (D) AlexaFluor488-labeled albumin (100 μM). Time constants determined for the positively and negatively charged PEGs were not significantly different. The 4-kD dextran equilibrates significantly slower, but fluorescent albumin diffuses quite rapidly. Similar to results for GFP (Fig. 7), however, the fluorescent albumin fluorescence remains substantially lower than pipette fluorescence. AFU, arbitrary fluorescence units.

Diffusion of four additional fluorescent molecules into the cytoplasm of patch-clamped murine myocytes. Same experimental conditions as Figs. 6 and 7. Dashed lines indicate the positions of the line scans shown at right. Yellow dots indicate the end of the myocyte in proximity to the pipette tip. (A) 2 kD FITC-labeled PEG with one amino group (100 μM). (B) 2 kD FITC-labelled PEG with one carboxyl group (100 μM). (C) 4.4 kD TRITC-labeled dextran (10 μM). (D) AlexaFluor488-labeled albumin (100 μM). Time constants determined for the positively and negatively charged PEGs were not significantly different. The 4-kD dextran equilibrates significantly slower, but fluorescent albumin diffuses quite rapidly. Similar to results for GFP (Fig. 7), however, the fluorescent albumin fluorescence remains substantially lower than pipette fluorescence. AFU, arbitrary fluorescence units.

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