Two i mages of a membrane-stained nephron (Invitrogen, FluoCells prepared slide #3). (A) Image taken on the microscope employed in all diffusion experiments described in this article: Nikon Eclipse TE2000 microscope equipped with Aurox Clarity and a LWD 40× 1.15 NA water immersion lens. (B) Image taken of the same slide with a Nikon CSU-W1 spinning disc confocal microscope equiped with a 40× 1.3 NA air lens and employing a Hamamatsu Orca Fusion camera. Confocality achieved with the Aurox Clarity system is at minimum comparable to the Nikon spinning disc system.