NCI-H295R cells are poorly ciliated. Quantification of the percentage of ciliated NCI-H295R and NIH3T3/Smo-mEos2 cells, counted as cells positive for each of the ciliary markers: acetylated tubulin (AcTUB), ARL13B, glutamylated tubulin (GlutTUB), or IFT88. The number of counted cells is given under the graph. Data are presented as mean ± SD, n = 6 replicates, pooled from three experiments. *, P < 0.05; **, P < 0.01.