HFD-induced obesity does not change the fractionation of SHH or lipoproteins in the adrenal gland. (A) Western blots of lysed adrenals isolated from mice fed a normal diet or a HFD, fractionated by differential centrifugation, probed for SHH and lipoprotein markers. (B) Western blots of density gradient fractions from adrenal supernatants at 16,000 g and 150,000 g of mice fed a normal diet or a HFD, probed for SHH and lipoprotein markers. Density of lipoprotein classes is according to Jonas and Phillips (2008). The experiment was repeated two times with similar results. VLDL, very-low-density lipoproteins.