Figure 7.
RY785 modifies gating currents. Kv2.1 gating currents were elicited in K+-free solutions with 100-ms steps to voltages between −80 and +80 mV from a holding potential of −100 mV (ON gating currents) and during subsequent 50-ms steps to −140 mV (OFF gating currents). (A) Exemplar ON gating currents at +40 mV before (black) and after (red) application of RY785. Integration windows used for the analysis in C (highlighted) were 0.2–6 ms after the voltage step. (B) Exemplar OFF gating currents at −140 mV following the steps shown in A before (black) and after (red) application of RY785. Integration windows used for the analysis in D (highlighted) were 0.2–20 ms after the voltage step. (C) Mean ON gating charge transfer (QON) as a function of voltage from n = 8 vehicle-treated cells (blue) and n = 4 RY785-treated cells (red). Mean ± SEM. *, P < 0.05. Curve is fit of Eq. 2 with x held at 1. Function variables ± SD, vehicle: Vhalf = −20.1 ± 5.8 mV, z = 1.62 ± 0.57 e0, A = 0.79 ± 0.11. RY785 data was not well fitted by Eq. 2. (D) Mean OFF gating charge (QOFF) transfer as a function of voltage from vehicle-treated cells (blue) and RY785-treated cells (red). Mean ± SEM. *, P < 0.05. Vehicle: n = 7 overall, ≥6 at each voltage; RY785: n = 4 overall, ≥2 at each voltage. Curve is a fit of Eq. 2 with x held at 1. Function variables ± SD: Vhalf = −34.4 ± 9.0 mV, z = 1.7 ± 1.0 e0, A = 0.84 ± 0.21. RY785 was not well fitted by Eq. 2. (E) Mean time constants of activation (τON) determined by fitting Eq. 4 to the decay phase of ON gating current traces from n = 8 vehicle-treated cells (blue) and n = 4 RY785-treated cells (red). Mean ± SEM. Lines are fits of Eq. 7. Function variables ± SD; vehicle, τ = 3.2 ± 0.6 ms, z = 0.52 ± 0.09 e0; RY785, τ = 2.7 ± 0.5 ms, z = 0.41 ± 0.09 e0. (F) Mean time constants of deactivation (τOFF) determined by fitting Eq. 4 to the decay phase of OFF gating current traces from vehicle-treated cells (blue) and RY785-treated cells (red). Mean ± SEM. *, P < 0.05. Vehicle, n = 8 overall, ≥6 at each voltage; RY785, n = 4 overall, ≥2 at each voltage.

RY785 modifies gating currents. Kv2.1 gating currents were elicited in K+-free solutions with 100-ms steps to voltages between −80 and +80 mV from a holding potential of −100 mV (ON gating currents) and during subsequent 50-ms steps to −140 mV (OFF gating currents). (A) Exemplar ON gating currents at +40 mV before (black) and after (red) application of RY785. Integration windows used for the analysis in C (highlighted) were 0.2–6 ms after the voltage step. (B) Exemplar OFF gating currents at −140 mV following the steps shown in A before (black) and after (red) application of RY785. Integration windows used for the analysis in D (highlighted) were 0.2–20 ms after the voltage step. (C) Mean ON gating charge transfer (QON) as a function of voltage from n = 8 vehicle-treated cells (blue) and n = 4 RY785-treated cells (red). Mean ± SEM. *, P < 0.05. Curve is fit of Eq. 2 with x held at 1. Function variables ± SD, vehicle: Vhalf = −20.1 ± 5.8 mV, z = 1.62 ± 0.57 e0, A = 0.79 ± 0.11. RY785 data was not well fitted by Eq. 2. (D) Mean OFF gating charge (QOFF) transfer as a function of voltage from vehicle-treated cells (blue) and RY785-treated cells (red). Mean ± SEM. *, P < 0.05. Vehicle: n = 7 overall, ≥6 at each voltage; RY785: n = 4 overall, ≥2 at each voltage. Curve is a fit of Eq. 2 with x held at 1. Function variables ± SD: Vhalf = −34.4 ± 9.0 mV, z = 1.7 ± 1.0 e0, A = 0.84 ± 0.21. RY785 was not well fitted by Eq. 2. (E) Mean time constants of activation (τON) determined by fitting Eq. 4 to the decay phase of ON gating current traces from n = 8 vehicle-treated cells (blue) and n = 4 RY785-treated cells (red). Mean ± SEM. Lines are fits of Eq. 7. Function variables ± SD; vehicle, τ = 3.2 ± 0.6 ms, z = 0.52 ± 0.09 e0; RY785, τ = 2.7 ± 0.5 ms, z = 0.41 ± 0.09 e0. (F) Mean time constants of deactivation (τOFF) determined by fitting Eq. 4 to the decay phase of OFF gating current traces from vehicle-treated cells (blue) and RY785-treated cells (red). Mean ± SEM. *, P < 0.05. Vehicle, n = 8 overall, ≥6 at each voltage; RY785, n = 4 overall, ≥2 at each voltage.

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