Rates of inhibition determined from single voltage steps. (A) Representative current decay during postpulses. First pulse in 1 µM RY785 (red) fitted with an exponential function (Eq. 4, gray curve). Function variables ± SD, I₀ = 34.6 ± 1.4 pA, τ = 0.19591 ± 0.00051 s. Average of 10 traces in vehicle (black) and fit (light gray curve), τ ± SD = 7.4 ± 1.2 s, I₀ held at 34.6 pA. (B) Category plot showing inhibition rates at three voltages. Hollow symbols represent rates of current decay in RY785 from individual cells. Filled symbols represent rates of current decay in RY785 corrected by subtracting rate of current decay in vehicle from the same cell. Stars correspond to the exemplar shown in A. Geometric means ± SEM of corrected rates are shown as horizontal lines. n = 4 or 5 per voltage.