RY785 blocks Kv2.1. Displayed currents in 1 μM RY785 are following a train of depolarizations to allow RY785 to inhibit Kv2.1 currents. (A) Top: Voltage command. Bottom: Representative currents from a whole-cell voltage-clamped Kv2.1-CHO cell in vehicle (black) and RY785 (red). (B) Current remaining during inhibition by RY785 from eight cells (red symbols). Horizontal line is mean ± SEM. (C) Current as a function of command voltage. Currents are means 98–100 ms after the start of the activating voltage step. Symbols correspond to individual cells in vehicle (black) and RY785 (red). Points are excluded if the predicted voltage error of clamp is >10 mV. Inset: Structure of RY785.