Cilium showing no remarkable fluorescent response. (A) Photomicrograph of a single ORC. The white square shows the ROI area where the UV stimulus was applied, and the fluorescence signal was measured. (B) The current response to a UV stimulus under scan mode; duration, 1.30 s. (C) Top: Analysis ROIs, red circles. Middle: Before UV (third plot in D). Bottom: During UV (fourth plot in D). Scaling Y, 0.18 µm. Scan speed, 1.30 s/scan. The intervals between image scan initiations were 4.03 s. Line sum, 2. (D) Change in fluorescence intensity (12-bit data); n = 8. Plots are means of fluorescence intensities obtained from analysis ROIs in the UV irradiation area (n = 8). The current response shown in B was obtained at the timing of UV in this panel. Error bars show the SD from those data.
Figure 5.

Cilium showing no remarkable fluorescent response. (A) Photomicrograph of a single ORC. The white square shows the ROI area where the UV stimulus was applied, and the fluorescence signal was measured. (B) The current response to a UV stimulus under scan mode; duration, 1.30 s. (C) Top: Analysis ROIs, red circles. Middle: Before UV (third plot in D). Bottom: During UV (fourth plot in D). Scaling Y, 0.18 µm. Scan speed, 1.30 s/scan. The intervals between image scan initiations were 4.03 s. Line sum, 2. (D) Change in fluorescence intensity (12-bit data); n = 8. Plots are means of fluorescence intensities obtained from analysis ROIs in the UV irradiation area (n = 8). The current response shown in B was obtained at the timing of UV in this panel. Error bars show the SD from those data.

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