Basal fluorescence. (A) Photomicrograph of a single ORC. The blurred recording pipette can be recognized on the right. The arrow indicates the target cilium. (B) Fluorescence image of a single cilium indicated in A. Line sum, 2. (C) Positions of analysis ROIs (colored circles, 0.41-µm diameter) used for fluorescence measurements. Scaling Y, 0.045 µm. Scan speed, 1.09 s/scan. (D) Relation between the basal fluorescence intensity (F₀, 12-bit resolution) and the distance from the knob. Data bars are the mean of six plots (technical replicates) during the time sequence and error bars the SD. (E) Superimposition of data from five different cells (biological replicates). Background and autofluorescence were not subtracted.