Previously described binding Sites I and II for LIN. (A) Sideview cartoon of KCNQ1 subunits S1–S6. (B) Top view of KCNQ1 with S1–S6 labeled and the voltage sensing domain (VSD) and pore domain (PD) circled. (C) Side and top view of binding site regions I and II for LIN. (D) Residues previously described in simulations to be important for LIN binding at Site I and II. Residues only suggested in simulation are shown in gray. Residues confirmed by mutation are shown in color. All simulation data and mutation data for L272, Y268, F269, A290, and L263 in this figure is from Yazdi et al. (2021). Mutation data for R218 and K316 is from Liin et al. (2018).