Nfil3 ^−/− mice exhibit defective NK cell populations. (A and B) Representative flow cytometric histograms of NK (A, NK1.1⁺CD3⁻; B, CD122⁺DX5⁺) and NK T (NK1.1⁺CD3⁺) cells present in the indicated organs from WT and Nfil3^−/− mice. Percentages of cells in each quadrant are representative of three mice analyzed per genotype. (C) Quantification of the percentages (left) and total numbers (right) of lymphocyte-gated NK and NK T cells in the indicated organs from WT and Nfil3^−/− mice. Data shown are the mean ± SEM of three animals analyzed per genotype. NK cell percentages and numbers were significantly reduced in Nfil3^−/− mice. *, P < 0.05; **, P < 0.01. Gating was identical to the FACS profiles shown in A and B.