[Ca²⁺]_c(t) and [Ca²⁺]_SR(t) in a WT cell with BAPTA. (A) Fluorescence of X-rhod-1. (B) R(x,t) and (in white trace) its line average R(t). (C) Plots of release flux and [Ca²⁺]_SR(t) as in Figs. 2 and 3. Internal solution was BAPTA. Note the radical changes in amplitude and kinetics of flux compared with Fig. 2, most notably an increase in peak and intermediate level, with a reduction in duration of the intermediate stage and the appearance of a hump (arrow). Also note a greatly expanded temporal scale, to better display the hastened kinetics. ID: 051209b _s43. Average of 13 line scan images taken 47–72 min after patching is shown.