Figure 3.
![Figure 3. Simultaneous determination of [Ca2+]c(t) and [Ca2+]SR(t) in a Casq1-null cell. (A) Fluorescence of cytosolic monitor in cell perfused with EGTA. (B) R(x,t) and (in white trace) its line average R(t). The dashed line marks Rmin. (C) Release flux (black) and [Ca2+]SR(t) (red). Details of description were given in legend of Fig. 2. Note the greatly increased degree of depletion by the end of the pulse. Also note the continued decrease of R(t) during the pulse, contrasting with stabilization in the WT (Fig. 2). ID: 062410a _s34. Average of eight line scans obtained 31–51 min after establishing whole-cell patch is shown.](https://cdn.rupress.org/rup/content_public/journal/jgp/138/2/10.1085_jgp.201010592/5/jgp_201010592_rgb_fig3.jpeg?Expires=1767782548&Signature=j7o5PeRYVbYywKLTSoZUyn8zaGZw63ZCKVjquafbaHGSj-AAoZbZEErG2Tn7IOmfJbRdDOYAnelnFpEXC5zaceozOxL2LOff-15PD7KbLxQ6wt4QAIQG9JdSA4b6wTOalSRmJFqia6jR7WWVxBn4xavqKJVuNw4s3SNQK~AUopGDrqHeCaLoQkU3XmQA7MGtCzzkpVkEQxMCUwJC7iQYia5DUhT-GL2yyPVrn6~luxwBIEXdoiriRAeW6tLZKrAJvHawFmTB0MNbEPtSVZdBZee7h9E-nbRGnwGpWm7BSGbUNfOrENldCz4AJ124Md9lNi704QVu6QATo7rOoDQXKQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Simultaneous determination of [Ca2+]c(t) and [Ca2+]SR(t) in a Casq1-null cell. (A) Fluorescence of cytosolic monitor in cell perfused with EGTA. (B) R(x,t) and (in white trace) its line average R(t). The dashed line marks Rmin. (C) Release flux (black) and [Ca2+]SR(t) (red). Details of description were given in legend of Fig. 2. Note the greatly increased degree of depletion by the end of the pulse. Also note the continued decrease of R(t) during the pulse, contrasting with stabilization in the WT (Fig. 2). ID: 062410a _s34. Average of eight line scans obtained 31–51 min after establishing whole-cell patch is shown.
