Conductance recovers quicker than [Cl⁻]_i and current. (A) Conductance, current, and [Cl⁻]_o/[Cl⁻]_i during two sequences of GABA application, separated by a recovery interval of 38 s, from one neuron. [Cl⁻]_i normalized to that before agonist application, current, and conductance to the values at the first ramp sequence, close to peak current during the first application. Note that conductance recovered fully during the 38-s interval, whereas there was only slight recovery of current and very little of [Cl⁻]_i. V_m = −14 mV; GABA concentration, 1.0 mM. (B) [Cl⁻]_i, normalized to that after 20 s in 1.0 mM GABA or 1.0 mM glycine, plotted versus the recovery interval without agonist. Lower dashed line corresponds to the [Cl⁻]_i (∼9 mM) before agonist application. The smooth curve is a fitted exponential with a time constant of 31 min. Data are from 10 neurons. (C and D) Conductance at a second application of 1.0 mM GABA (C) or 1.0 mM glycine (D), normalized to that at peak current during a first 20-s agonist application and plotted versus the recovery interval. Smooth curves are fitted exponentials with time constants of 13 s (C) and 14 s (D). Data are from eight (C) and three (D) neurons. Gramicidin-perforated patch mode used in A–D.