Various insertion mutants before or after the core domain do not affect force activation. (A and C) Diagrams showing insertions with different sequences, different lengths, and different locations. Insertion mutants with no observed functional channels are not depicted in the diagram, which include 369-6GGS, 372-6GGS, 373-6GGS, 375-6GGS, 376-6GGS, 377-6GGS, and 378-6GGS. (B and D) Similar diagrams and recording as in Fig. 3 of 486-11QTL and 358-24GGS, respectively.