Figure 9. Estimation of the diffusion coefficient of PAGFP in the myoid. (A; left) x–y image of the region of retinal slice showing the z level of the cell on which the experiment was performed. The region of the myoid that was exposed to a 100-µs photoconversion pulse is indicated by the red symbol. The green line indicates the location of 2-KHz line scans that intersected the photoconversion site. (Right) The spatiotemporal fluorescence map of the dissipation of the photoconverted PAGFP from the photoconversion site. (B) Fluorescence profiles of selected line scans after normalizing to the amplitude of the first post-pulse line scan recorded (1.176 ms). The red lines are the result of model calculations with optimal D. (C) Root mean square (RMS) error calculated from the difference between line scan fluorescence and model prediction for indicated D. Minimal ERMS was achieved with D = 5 µm2 s−1.
Figure 9.

Estimation of the diffusion coefficient of PAGFP in the myoid. (A; left) x–y image of the region of retinal slice showing the z level of the cell on which the experiment was performed. The region of the myoid that was exposed to a 100-µs photoconversion pulse is indicated by the red symbol. The green line indicates the location of 2-KHz line scans that intersected the photoconversion site. (Right) The spatiotemporal fluorescence map of the dissipation of the photoconverted PAGFP from the photoconversion site. (B) Fluorescence profiles of selected line scans after normalizing to the amplitude of the first post-pulse line scan recorded (1.176 ms). The red lines are the result of model calculations with optimal D. (C) Root mean square (RMS) error calculated from the difference between line scan fluorescence and model prediction for indicated D. Minimal ERMS was achieved with D = 5 µm2 s−1.

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