PAGFP equilibration in the myoid is isotropic and rapid. (A) x–y image of the region of retinal slice at the central z level of the cell that was the subject of the experiment. The region of the cell that was rapidly scanned before and after a 100-µs photoconversion pulse (the location of which is in the myoid, indicated by the red symbol) is delineated by the green box. (B) Pre-conversion scan of the region showing subregions where time courses of fluorescence change were recorded (red boxes). (C) Selected time course images showing the rapid myoid equilibration. (D) Time courses of fluorescence changes recorded from the regions shown in B. Note that the axial and radial regions change in parallel and ultimately merge with the fluorescence time course from region 1, the site of photoconversion, at ∼1.5 s. See Video 2.