Figure 4.
Figure 4. Modification of introduced cysteines by pretreatment with external MTSET. (A) Example leak-subtracted I-V relationships for each of the four MTSET-sensitive mutants named, showing the effects of the application of internal MTSET (2 mM) after maximal channel activation with 20 nM PKA, 1 mM ATP, and 2 mM PPi. Patches were excised from cells that had been pretreated with external MTSET (5 mM for 5 min) and showed similar sensitivity to internal MTSET as patches excised from untreated cells (see Fig. 1 B for examples). (B) Comparison of the effects of MTSET on macroscopic current amplitude at −80 mV between patches from untreated cells and patches from cells pretreated with external MTSET. There were no statistically significant differences for any mutant studied (P > 0.05). Mean of data from three to nine patches is shown.

Modification of introduced cysteines by pretreatment with external MTSET. (A) Example leak-subtracted I-V relationships for each of the four MTSET-sensitive mutants named, showing the effects of the application of internal MTSET (2 mM) after maximal channel activation with 20 nM PKA, 1 mM ATP, and 2 mM PPi. Patches were excised from cells that had been pretreated with external MTSET (5 mM for 5 min) and showed similar sensitivity to internal MTSET as patches excised from untreated cells (see Fig. 1 B for examples). (B) Comparison of the effects of MTSET on macroscopic current amplitude at −80 mV between patches from untreated cells and patches from cells pretreated with external MTSET. There were no statistically significant differences for any mutant studied (P > 0.05). Mean of data from three to nine patches is shown.

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