Figure 2.
Figure 2. NFA activates Slo2.1 channels heterologously expressed in oocytes. (A) Currents recorded from an uninjected oocyte before and after treatment with 1 mM NFA. (B) Currents recorded from an oocyte expressing Slo2.1 before (control) and after treatment with 1 mM NFA for 8 min. Oocytes were injected 2 d earlier with 0.32 ng Slo2.1 cRNA. In A and B, Vt was varied from −160 to +80 mV, applied in 20-mV increments. (C) Average I-V relationships for currents recorded before (control) and after treatment with 1 mM NFA (n = 35). (D) [NFA]– and [FFA]–response relationships for increase of ISlo2.1 in response to continuous pulsing to 0 mV. Data were fitted with a Hill equation (smooth curves) to determine EC50 (2.08 ± 0.04 mM) and Hill coefficient, nH (1.94 ± 0.05), for NFA (n = 12). For FFA, the EC50 was 1.4 ± 0.02 mM and nH was 1.92 ± 0.03 (n = 5).

NFA activates Slo2.1 channels heterologously expressed in oocytes. (A) Currents recorded from an uninjected oocyte before and after treatment with 1 mM NFA. (B) Currents recorded from an oocyte expressing Slo2.1 before (control) and after treatment with 1 mM NFA for 8 min. Oocytes were injected 2 d earlier with 0.32 ng Slo2.1 cRNA. In A and B, Vt was varied from −160 to +80 mV, applied in 20-mV increments. (C) Average I-V relationships for currents recorded before (control) and after treatment with 1 mM NFA (n = 35). (D) [NFA]– and [FFA]–response relationships for increase of ISlo2.1 in response to continuous pulsing to 0 mV. Data were fitted with a Hill equation (smooth curves) to determine EC50 (2.08 ± 0.04 mM) and Hill coefficient, nH (1.94 ± 0.05), for NFA (n = 12). For FFA, the EC50 was 1.4 ± 0.02 mM and nH was 1.92 ± 0.03 (n = 5).

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