PIP 5-kinase overexpression antagonizes VSP effects. (A) Traces from two cells with similar current amplitudes transfected with Dr-VSP and KCNQ2/3 (ctrl., black trace) or with Dr-VSP, KCNQ2/3, and PIP 5-kinase Iγ (+5K, red trace). (B) Dependence of current inhibition on the duration of VSP activation. Baseline-normalized currents at −20 mV immediately after VSP activation are plotted for control (from Fig. 3 D) and +5K (three cells). (C) Time constants of single-exponential fits to current recovery after VSP activation. Summary of 16 cells for control and 4 cells for +5K.