Figure 12.
Figure 12. Colocalization of IYFP with net FRET and NFRET signals. (A) Line profile analysis of YFP fluorescence. nF and NFRET measurements through the mGAT1XFP8-expressing cell in B illustrate the peripheral localization of the signals for all parameters. (B) Images report the colocalization of YFP-expressing pixels with the nF or NFRET signals. Correlation of the nF or NFRET signal with YFP intensity is plotted in the bottom panels. Note that the normalization routine allows NFRET to measure FRET independently of YFP intensity.

Colocalization of IYFP with net FRET and NFRET signals. (A) Line profile analysis of YFP fluorescence. nF and NFRET measurements through the mGAT1XFP8-expressing cell in B illustrate the peripheral localization of the signals for all parameters. (B) Images report the colocalization of YFP-expressing pixels with the nF or NFRET signals. Correlation of the nF or NFRET signal with YFP intensity is plotted in the bottom panels. Note that the normalization routine allows NFRET to measure FRET independently of YFP intensity.

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