Expression and subcellular localization of C-terminal XFP addition mGAT1 constructs in N2a cells. Each panel displays representative fluorescence microscopy images of live N2a cells expressing each C-terminal addition construct 48 h after transfection using the linear range parameters described in Materials and methods. The nomenclature for each construct has been abbreviated to remove the “mGAT1” because this element is common to all the constructs displayed (i.e., mGAT1₀GFP = ₀GFP and mGAT1YFP8 = YFP8). Bars, 10 µm.