Figure 11.
The half-time of translocation as a function of voltage. The values for WT LFN (circles) and His6-LFN (triangles) are from the experiments described in Fig. 9. Note that at every voltage, the rate of translocation of His6-LFN is slower than that of WT LFN but that the rates tend to converge as the voltage increases. Thus, His6-LFN t1/2/WT LFN t1/2 = 3.3, 2.3, 1.6, and 1.1 for V = 45, 50, 55, and 60 mV, respectively. The fitted curves were calculated from the drift-diffusion model as described in the Results section.
