![Figure 7. Changes in [Ca2+]o sensitivity of TRPM2 mutants H958Q, D964N, and E994Q. (A–D) Inward and outward currents of WT TRPM2 (A), H958Q (B), D964N (C), and E994Q (D) activated by the indicated [Ca2+]o. (E) Mean current amplitude of WT TRPM2, H958Q, D964N, and E994Q at the indicated [Ca2+]o. (F) Concentration-dependent effects of [Ca2+]o. Currents were normalized to the maximal value obtained at 2 mM [Ca2+]o. EC50s for Ca2+-mediated activation were 131 ± 10.8 µM (nH = 3.3 ± 0.5; n = 8) for WT TRPM2, 239 ± 25 µM (nH = 2.7 ± 0.5; n = 8) for H958Q, 256 ± 30.2 µM (nH = 2.8 ± 0.4; n = 8) for D964N, and 298 ± 52.6 µM (nH = 2.6 ± 0.4; n = 8) for E994Q.](https://cdn.rupress.org/rup/content_public/journal/jgp/134/6/10.1085_jgp.200910254/6/jgp_200910254_rgb_fig7.jpeg?Expires=1767685405&Signature=YwdS7q7G-WViWkDJbphvYzXoRsK8j5lDhYrZ0KxEqO60I7XeVLLppL-RKcBzxxtH1zh-cZNM7IBvus8skJI5ZxnYey~OLSF7IVuiZCggI1TjVj1Jdy2Soy8sMLpz7amG0WVY8aOypX1PDM3FvGT0vZSsXVLELz8SQq43s1z2HH7kwMEaCKuMbxS6Tis8XX1mLR0MwltkYS11hPIaDjOp~B135iNWP-L~~K6R3NJhIJDbqVYfxjUyCCy0di5Pp4MVgw6T-gvLahIJYE46yEUpl3RWdpDcWG-VRQSGVXFLNAi0eGSeJz4epFD~ZTQ9TbslDR9TdMDUeWcW4~-veuGP8g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 7.
Changes in [Ca2+]o sensitivity of TRPM2 mutants H958Q, D964N, and E994Q. (A–D) Inward and outward currents of WT TRPM2 (A), H958Q (B), D964N (C), and E994Q (D) activated by the indicated [Ca2+]o. (E) Mean current amplitude of WT TRPM2, H958Q, D964N, and E994Q at the indicated [Ca2+]o. (F) Concentration-dependent effects of [Ca2+]o. Currents were normalized to the maximal value obtained at 2 mM [Ca2+]o. EC50s for Ca2+-mediated activation were 131 ± 10.8 µM (nH = 3.3 ± 0.5; n = 8) for WT TRPM2, 239 ± 25 µM (nH = 2.7 ± 0.5; n = 8) for H958Q, 256 ± 30.2 µM (nH = 2.8 ± 0.4; n = 8) for D964N, and 298 ± 52.6 µM (nH = 2.6 ± 0.4; n = 8) for E994Q.
