Figure 2. Effects of external protons on single-channel conductance. Single-channel currents were recorded under inside-out configuration with external solutions (pipette solutions) containing 2 mM Ca2+ and internal solutions (bath solutions) containing nominal Ca2+-free Tyrode's solution and 200 µM ADPR (pHo 7.2). (A) Representative recording of TRPM2 at +80 mV with the external solution (pipette solution) at pHo 7.4. (B) Single-channel recordings of TRPM2 at +80 mV with the external solution (pipette solution) at pHo 5.5. (C) Average current amplitude at each voltage plotted against the test potential. Linear regression yielded single-channel conductances of 75.4 ± 0.8 pS (mean ± SEM; n = 7∼9) at pHo 7.4 and 43.2 ± 0.9 pS (mean ± SEM; n = 7∼9) at pHo 5.5.
Figure 2.

Effects of external protons on single-channel conductance. Single-channel currents were recorded under inside-out configuration with external solutions (pipette solutions) containing 2 mM Ca2+ and internal solutions (bath solutions) containing nominal Ca2+-free Tyrode's solution and 200 µM ADPR (pHo 7.2). (A) Representative recording of TRPM2 at +80 mV with the external solution (pipette solution) at pHo 7.4. (B) Single-channel recordings of TRPM2 at +80 mV with the external solution (pipette solution) at pHo 5.5. (C) Average current amplitude at each voltage plotted against the test potential. Linear regression yielded single-channel conductances of 75.4 ± 0.8 pS (mean ± SEM; n = 7∼9) at pHo 7.4 and 43.2 ± 0.9 pS (mean ± SEM; n = 7∼9) at pHo 5.5.

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