Figure 1. Inhibitory effects of acidic pHo on TRPM2. (A) Both inward and outward currents of TRPM2 elicited by voltage ramps ranging from −100 to +100 mV were completely and reversibly inhibited by pHo 4.5. Note that pHo 4.0 irreversibly blocked TRPM2. Internal pipette solutions contained 100 nM [Ca2+]i buffered by 1 mM EGTA and 200 µM ADPR. Inward and outward currents were measured at −100 and +100 mV, respectively. (B) Representative recordings of TRPM2 by ramp protocols ranging from −100 to +100 mV at the indicated pHo. (C) Mean current amplitude of TRPM2 at the indicated pHo (mean ± SEM; n = 9). (D) Dose–response curve of pHo constructed by normalizing current amplitude at the indicated pHo to the maximal current amplitude at pH7.4. Best fit of the normalized data yielded an IC50 of 5.3 ± 0.05 pH units (mean ± SEM; n = 9; Hill coefficient, nH = 1.3).
Figure 1.

Inhibitory effects of acidic pHo on TRPM2. (A) Both inward and outward currents of TRPM2 elicited by voltage ramps ranging from −100 to +100 mV were completely and reversibly inhibited by pHo 4.5. Note that pHo 4.0 irreversibly blocked TRPM2. Internal pipette solutions contained 100 nM [Ca2+]i buffered by 1 mM EGTA and 200 µM ADPR. Inward and outward currents were measured at −100 and +100 mV, respectively. (B) Representative recordings of TRPM2 by ramp protocols ranging from −100 to +100 mV at the indicated pHo. (C) Mean current amplitude of TRPM2 at the indicated pHo (mean ± SEM; n = 9). (D) Dose–response curve of pHo constructed by normalizing current amplitude at the indicated pHo to the maximal current amplitude at pH7.4. Best fit of the normalized data yielded an IC50 of 5.3 ± 0.05 pH units (mean ± SEM; n = 9; Hill coefficient, nH = 1.3).

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