Figure 6.
![Figure 6. Properties of stationary currents of ATP1A2 mutants YY-FF and YY-AA. (A and B) Normalized I-V curves for NaIxK currents of ATP1A2 mutants YY-FF (A) and YY-AA (B), with [K+]ext as indicated ([Na+]ext = 100 mM, pH 7.4). (C and D) NaK0.5(K+ext) values from NaIxK currents for mutant YY-FF (C) and YY-AA (D) from fits of a Hill function to the data in A and B at each membrane potential. The corresponding WT data from Fig. 2 E (dashed lines in C and D) and ΔYY data from Fig. 2 C (dotted lines in C and D) are superimposed. (E) I-V curves of normalized ouabain-sensitive NaI(ouab) currents ([Na+]ext = 100 mM and [K+]ext = 0, pH 7.4) for ATP1A2 WT and mutants YY-FF, YY-AA, and ΔYY. Data are means ± SE of 10–14 cells from three batches.](https://cdn.rupress.org/rup/content_public/journal/jgp/135/2/10.1085_jgp.200910301/7/jgp_200910301_lw_fig6.jpeg?Expires=1767780957&Signature=1ezZ~rSYH6Ybm6KKRzoPOiOBX9QddFMk8zPtU1eVMeUaC3kyT0S7asLQNAqQwY58bxCKJpJ5T1dUY7agLavsZog~2CY2QJtN9-70pHmZZrDYEM~L~ABlQwyDfsUvVOulOy5yRGQn6t0pIDAV3fDpB-88DKlcXjNbVIFzk2zM0mEH23qTpwGZCFrLQyltR4wIkuzAYBJt4jS8G6KKKOwcNrT4BMjKLsBI29XGuVJUSf9aF7AtdYEE~ptdr0iLfdKiX21v~aNGS5I4mjnNYN-rWvvQ~g88F41NykoqkHOqlXl3Gjf~bOWOXh4AncU5KHRUY5SKGNQuLxbM2qKH9LYG4w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Properties of stationary currents of ATP1A2 mutants YY-FF and YY-AA. (A and B) Normalized I-V curves for NaIxK currents of ATP1A2 mutants YY-FF (A) and YY-AA (B), with [K+]ext as indicated ([Na+]ext = 100 mM, pH 7.4). (C and D) NaK0.5(K+ext) values from NaIxK currents for mutant YY-FF (C) and YY-AA (D) from fits of a Hill function to the data in A and B at each membrane potential. The corresponding WT data from Fig. 2 E (dashed lines in C and D) and ΔYY data from Fig. 2 C (dotted lines in C and D) are superimposed. (E) I-V curves of normalized ouabain-sensitive NaI(ouab) currents ([Na+]ext = 100 mM and [K+]ext = 0, pH 7.4) for ATP1A2 WT and mutants YY-FF, YY-AA, and ΔYY. Data are means ± SE of 10–14 cells from three batches.
