Figure 4.
Figure 4. Neutralization of D646 partially relieves proton block. (A) Representative single-channel current traces from the D646N mutant at different extracellular pHs. (Top row) Outward currents at +60 mV. (Bottom row) Inward currents at −60 mV. Solutions were symmetrical and contained 1 M KCl. (B–E) Plots of unitary current amplitudes versus ionic concentrations for the mutant channel at the indicated pHo (B, −60 mV; C, +60 mV). For comparison, similar plots for wild type are displayed in D and E.

Neutralization of D646 partially relieves proton block. (A) Representative single-channel current traces from the D646N mutant at different extracellular pHs. (Top row) Outward currents at +60 mV. (Bottom row) Inward currents at −60 mV. Solutions were symmetrical and contained 1 M KCl. (B–E) Plots of unitary current amplitudes versus ionic concentrations for the mutant channel at the indicated pHo (B, −60 mV; C, +60 mV). For comparison, similar plots for wild type are displayed in D and E.

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