Modulation of Bdel1 current by SP is modestly disrupted. HEK-M1 cells were transiently transfected with NK-1R, Bdel1, α₂δ-1, β2a (A–C), or β3 (D and E). (A) Averaged current–voltage plots measured before and after application of SP (red). (B and C) Individual sweeps elicited at 20 mV (B) and summary of enhancement (C) taken before and 2 min after application of SP (red) before (P1) or after a prepulse (P2; n = 7). (D) Representative sweeps taken before and 2 min after application of SP. (E) Summary of the inhibition by SP at 20 mV caused by SP before (P1) and after a prepulse (P2; n = 4); *, P < 0.05 compared with control currents. Error bars represent SEM. Bars, 10 ms and 200 pA.