Palmitoylation of β2a interferes with AA CaV1.3b inhibition. (A) Summary bar graphs of percent inhibition of CaV1.3b currents with β2a, depalmitoylated β2a C3,4S (from Fig. 10 A for comparison), β2a C3,4S in the presence of 10 µM PA for 1 min, and β2a C3,4S preincubated in 10 µM PA for at least 8 min, and then exposed to 10 µM PA + µM AA for 1 min (n = 3–4; *, P < 0.05 compared with β2a C3,4S). (B) Representative current traces. (Left, top) CaV1.3b coexpressed with depalmitoylated β2a C3,4S before (CTL) and after exposure to 10 µM PA for 1 min. (Left, bottom) β2a C3,4S preincubated with 10 µM PA for 8 min (PA), and the addition of 10 µM PA + 10 µM AA for 1 min (AA). (Right) Normalized to the end of the 40-ms test pulse. (C) Summary bar graphs of current remaining for each condition. Bars, 0.5 nA, 10 ms.
