AA does not effect holding potential–dependent inactivation of CaV1.3b currents. (A) Representative current traces from CaV1.3b, β2a channels for CTL conditions (top) or 1 min after a 10 µM AA application (bottom) from a holding potential of −60 mV to conditioning pulses from −40 to +30 mV for 2.2 s, and then to a test pulse at −10 mV for 40 ms. Bar, 0.2 nA. (B) Representative current traces from CaV1.3b, β3 channels. Conditions are similar to A. Bar, 0.5 nA. (C) Representative current traces from CaV1.3b, β2a channels for CTL (gray) or inhibited currents 1 min after a 10-µM AA application (light gray) for 2.2-s conditioning pulses from −20 to +30 mV. Fits of inactivation overlay each trace (dark gray, CTL; red, AA). The 40-ms test pulse after the conditioning pulse is expanded on the right. Bar, 0.2 nA. (D) Representative current traces from CaV1.3b, β3 channels. Conditions are similar to C. Bar, 0.5 nA.
