Figure 2.
Figure 2. Sensing currents from oocytes expressing different mutants of Ci-VSP. Sensing currents were recorded by applying voltage pulses of 400 ms, ranging from −100 to 140 mV. The HP was −60 mV. (A) Sensing currents for the phosphatase-inactivated mutant Ci-VSP C363S. When pulsing to positive potentials from the HP, all the mutants, R253A-R254A-C363S (B), R245Q-R246Q-C363S (C), and R245A-R246A (D), displayed sensing currents similar to those observed with the mutant C363S. In contrast, during the repolarization to the HP, all the PBM mutants displayed faster OFF sensing currents.

Sensing currents from oocytes expressing different mutants of Ci-VSP. Sensing currents were recorded by applying voltage pulses of 400 ms, ranging from −100 to 140 mV. The HP was −60 mV. (A) Sensing currents for the phosphatase-inactivated mutant Ci-VSP C363S. When pulsing to positive potentials from the HP, all the mutants, R253A-R254A-C363S (B), R245Q-R246Q-C363S (C), and R245A-R246A (D), displayed sensing currents similar to those observed with the mutant C363S. In contrast, during the repolarization to the HP, all the PBM mutants displayed faster OFF sensing currents.

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