Dialysis of 1.66 mM NADPH into rods before a 50% bleach of the visual pigment. (A) Changes in log sensitivity after pigment bleaching plotted as a function of time. Sensitivity before (upward filled triangle) and after (upward open triangle) dialysis with internal solution containing 1.66 mM NADPH (black bar) is plotted (mean ± SEM). A 50% bleach is given at time 0 s. Recovery of sensitivity at the base of the outer segment commenced with a time constant of ∼260 s, and at the tip of the outer segment of ∼950 s. (B) Suction electrode configuration while dialyzing 1.66 mM NADPH into the rod inner segment. On the bottom, responses to saturating flashes at selected time points during recovery in A are shown. (C) Dim flash responses in the dark-adapted cell after dialysis (gray) and at steady state after pigment bleaching (black) indicating the response speeding characteristic of bleaching adaptation.