Summary of SLC26A9 current time course and blocker effects. (A) HEK 293 cells treated with 50 µM GlyH-101 were significantly inhibited after 3 min and exhibited a partial recovery after 3 min of blocker washout. The effect of 100 µM glibenclamide was not statistically different from the 20% rundown in constitutive current normally observed in untreated cells. (B) The addition of forskolin during the blocker protocols did not significantly affect either the rundown or blocker efficacy. All currents are normalized to their respective basal current (see Results) at break-in. *, P < 0.05 compared against the same time point from unblocked cells and for paired t test with prior time point. Number of experiments for each case is indicated on the bar groups. A representative time course and protocol are shown in Fig. 2 A.