Figure 1. Characterization of the solution exchange. The recording pipette contained the normal pipette solution described in Materials and methods. (A) Schematic diagram showing the solution exchange by moving the solution-delivery pipes of the SF-77 rapid solution exchanger. Diameters of the solution-exchange pipe and the electrode tip are not proportionally drawn. Dotted lines indicate the junction plane of laminar flows. (B) Solution exchange characterized by measuring the junction current. The bath solution was changed from 100 mM KCl to 100 mM NaCl. Red line indicates the input of the command voltage signal to the SF-77 controller, and the black curve is the recorded current. The delay from the start of the voltage signal to the start of the current change was 19.5 ± 0.2 ms, and the time constant of the actual solution exchange was 0.95 ± 0.03 ms (n = 4).
Figure 1.

Characterization of the solution exchange. The recording pipette contained the normal pipette solution described in Materials and methods. (A) Schematic diagram showing the solution exchange by moving the solution-delivery pipes of the SF-77 rapid solution exchanger. Diameters of the solution-exchange pipe and the electrode tip are not proportionally drawn. Dotted lines indicate the junction plane of laminar flows. (B) Solution exchange characterized by measuring the junction current. The bath solution was changed from 100 mM KCl to 100 mM NaCl. Red line indicates the input of the command voltage signal to the SF-77 controller, and the black curve is the recorded current. The delay from the start of the voltage signal to the start of the current change was 19.5 ± 0.2 ms, and the time constant of the actual solution exchange was 0.95 ± 0.03 ms (n = 4).

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