Figure 8.
Figure 8. The double mutation T449V-I470C in one wt subunit impairs slow inactivation in 1wt/3mut, but not in 4wt. (A) Time course of slow inactivation. Representative current traces from oocytes expressing Shaker IR TVIC injected as monomers (left), wtTVIC/3wt (middle), and wtTVIC/3mut (right) from an HP of −90 mV to voltages from −20 to +60 mV in 20-mV intervals for 100 s. Cut-open voltage clamp measurements. The internal and external solutions contained 120 and 12 mM K+, respectively. (B) Ratio of the remaining current after a 100-s depolarizing pulse over the peak current (I100s/Ipeak) for Shaker TVIC injected as monomers (open black squares), 4wt (filled black squares), wtTVIC/3wt (filled red squares), 1wt/3mut (filled black circles), and wtTVIC/3mut (filled red circles).

The double mutation T449V-I470C in one wt subunit impairs slow inactivation in 1wt/3mut, but not in 4wt. (A) Time course of slow inactivation. Representative current traces from oocytes expressing Shaker IR TVIC injected as monomers (left), wtTVIC/3wt (middle), and wtTVIC/3mut (right) from an HP of −90 mV to voltages from −20 to +60 mV in 20-mV intervals for 100 s. Cut-open voltage clamp measurements. The internal and external solutions contained 120 and 12 mM K+, respectively. (B) Ratio of the remaining current after a 100-s depolarizing pulse over the peak current (I100s/Ipeak) for Shaker TVIC injected as monomers (open black squares), 4wt (filled black squares), wtTVIC/3wt (filled red squares), 1wt/3mut (filled black circles), and wtTVIC/3mut (filled red circles).

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