Figure 5.
![Figure 5. Introducing an SO3− at residues 1, 77, or 263 (by reacting the corresponding LFN cysteine mutant with MTS-ES; red) had very little or no effect on the translocation rate of LFN. The experiments were performed as described in Fig. 3. Note that if the nearby lysines were mutated to glutamates at positions K75E and K80E (green), an SO3− at position 77 greatly retarded LFN translocation as in Fig. 4. The glutamate mutations alone did not affect the translocation rate (not depicted). (For unknown reasons, the rate of translocation of LFNA1C reacted with MTS-ES, MTS-ET, or MTS-ACE [half-time of 10 s] was half that of WT LFN [half-time of 5 s].)](https://cdn.rupress.org/rup/content_public/journal/jgp/133/3/10.1085_jgp.200810170/6/jgp_200810170_rgb_fig5.jpeg?Expires=1767742549&Signature=mbFEzGn4EOxHt4pILXogV2MaP7EBou8q2jx3u18AccF30sdnoWaLXBBJwI8lXdBsxV24SreX5r89K6iwOAgmHrtjn0-soaoOmgpF-b2rs8kpZM1zYX3zxvrRwmGx9HBYvCNzuy7hDicmm9OdLaN8cn5diKo2g2E1rdZhMe4a84ZYf5GOEijAU-GjitxfruahEuRZfFQOcnZhqMpBMwZFlQ1KeOUPsbdAEJfPD1Kj-Yy98V0kEKsjypIUg0C0pxIPI91l6jqXmmm4JrvrhmgPMQ1yT6pteGbj7HU6GkXMQMCQA6Ir5TbuKX7ChFlzkTTI0NJgL6qHRvfTeMXeSu2lvg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Introducing an SO3− at residues 1, 77, or 263 (by reacting the corresponding LFN cysteine mutant with MTS-ES; red) had very little or no effect on the translocation rate of LFN. The experiments were performed as described in Fig. 3. Note that if the nearby lysines were mutated to glutamates at positions K75E and K80E (green), an SO3− at position 77 greatly retarded LFN translocation as in Fig. 4. The glutamate mutations alone did not affect the translocation rate (not depicted). (For unknown reasons, the rate of translocation of LFNA1C reacted with MTS-ES, MTS-ET, or MTS-ACE [half-time of 10 s] was half that of WT LFN [half-time of 5 s].)
