Lack of inward NMDG⁺ currents in cells expressing Kv3.2b P468W channels. (A) Gating current recording from transiently expressed Kv3.2b P468W channels using the pulse protocol shown at the top of A. The interpulse interval was 5 s. (Insets) Sequence of S6 helix of the Kv3.2b subunit with the mutated proline in bold. (B) The time courses of the charge movement during the pulses (top) and upon repolarization (bottom) for Kv3.2b P468W channels. The amount of charge (Q_on and Q_off) was calculated by integrating the currents during depolarizations and repolarizations shown in A. (C) Time constants for decay of the inward tail currents as a function of pulse potential from data as in A. (D) The ratio of Q_off/Q_on as a function of pulse potential from data as in A.