Figure 3.
![Figure 3. Mutating all three types of Ca2+ binding sites eliminates the Ca2+ dependence of Popen. Dose–response relationships for the effect of Ca2+ on Popen at 0 mV were obtained by plotting the mean log ratio of NPopen in the presence and absence of Ca2+ for ΔEΔRΔB (open circles) and ΔEΔRΔB + β1 (filled circles) channels. Each point represents the average between 7 and 11 patches at each [Ca2+] tested. Error bars represent SEM.](https://cdn.rupress.org/rup/content_public/journal/jgp/133/2/10.1085_jgp.200810129/6/jgp_200810129_lw_fig3.jpeg?Expires=1773593505&Signature=iXM~102IgbMvUFyD-a0sQAYii0Lb2bfbjQ~SKSKmGmBRM8EuSAFE2zC-W7EvrX3S3wTdGPbKlz6llaWXHVwPVkY-W8A5x1jsLcv4fKK8Pj0madDb6zBGYUbyiEvfEcdBkKNsH30OLNpqv1FsaA6w9QbwXoYcn7EFxbSYs-JBbeIZTPZfUddTtld5a379KVAH8ECJ-fPz0etOzWdVQEFJLqb7DOl3UOz9km4iHrt0-gFd-hm9qrXPdS9oohV0OmTkmlCg2WW9JK0cCrzhaCAXJ~mAqJAFjztmxNn3iXCsZ8-rMdgGGFWu62f~WZkTgOvBijrihQyy1BxhvNH3L0Oo5A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Mutating all three types of Ca2+ binding sites eliminates the Ca2+ dependence of Popen. Dose–response relationships for the effect of Ca2+ on Popen at 0 mV were obtained by plotting the mean log ratio of NPopen in the presence and absence of Ca2+ for ΔEΔRΔB (open circles) and ΔEΔRΔB + β1 (filled circles) channels. Each point represents the average between 7 and 11 patches at each [Ca2+] tested. Error bars represent SEM.
