Kinetic analysis with a simple two-state model. (A) Two-state model for P2X channel gating. C represents a closed state, and O represents an open state. α and β represent the transition rates between the closed and open states. (B) Representative α and β at various [ATP] were plotted versus membrane potentials. α and β were calculated from the data in Figs. 2 and 3 using two equations: G/Gmax = β/(α + β) and τ = 1/(α + β). (C) α and β in B were replotted versus [ATP].