Figure 4.
![Figure 4. Excised inside-out patch clamp recordings of the macroscopic current through P2X2 WT expressed in HEK293T cells with intensive perfusion of the bath (intracellular side) solution. (A) Macroscopic currents were recorded by inside-out patch clamp from HEK293T cell using the same voltage step protocols in Fig. 2 in the presence of the indicated [ATP]. Current traces recorded at 1 min (left) after excising the patch membrane and at 15 min (right) after an intensive perfusion by the blocker-free solution described in Materials and methods. (B) Dependence of the activation kinetics on voltage and [ATP].](https://cdn.rupress.org/rup/content_public/journal/jgp/133/1/10.1085_jgp.200810002/7/jgp_200810002_lw_fig4.jpeg?Expires=1770273349&Signature=xJZLxuivzLBYSUi7u8IwmQBJLXjhEueJxCg-BMPkn-s85nhkObSqA-wjDiWC0pFgK9UiFZmACoKRD2omeHan5481rt6V0MVA1nFX-cRqv4YiiiI2u8LOlRuzj5CGEvyNxPhc--1wL~EhuV~-PZPTjUK5-3YQXlQziwZhSTFFjWlmKFOY6CtVeXY2iesPRQhN5pADz0XuGPMmb7HmzGIZ4htlGGBpejRuiDCd6ezt31pZOjiaKubstCeMKEZyK4xV-P7nbwfR3t2cHIDo7U~v29vdd7vJQFIYYFnjOZPB0MRfSVpFs0sJJglBIR47GEP2vzV5nlhcFmo~xBCxDCHPTA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Excised inside-out patch clamp recordings of the macroscopic current through P2X2 WT expressed in HEK293T cells with intensive perfusion of the bath (intracellular side) solution. (A) Macroscopic currents were recorded by inside-out patch clamp from HEK293T cell using the same voltage step protocols in Fig. 2 in the presence of the indicated [ATP]. Current traces recorded at 1 min (left) after excising the patch membrane and at 15 min (right) after an intensive perfusion by the blocker-free solution described in Materials and methods. (B) Dependence of the activation kinetics on voltage and [ATP].
