Figure 2.
![Figure 2. Inhibition of RYR2 activity by luminal Mg2+. The traces were taken from a single experiment, and the luminal [Mg2+] is shown at the left of each trace. The cytoplasmic bath contained 2 mM ATP and 0.1 μM Ca2+ and did not contain any Mg2+. The luminal bath contained 0.1 mM Ca2+. Cytoplasmic and luminal baths also contained 230 mM CsCH3O3S and 20 mM CsCl, pH 7.4. Recordings obtained at +40 mV (top four traces) show channel openings as upward current jumps, whereas recordings at −40 mV show channel openings as downward current jumps.](https://cdn.rupress.org/rup/content_public/journal/jgp/132/4/10.1085_jgp.200810001/7/jgp_200810001_lw_fig2.jpeg?Expires=1773580053&Signature=BVbdKdXGBUAHPkbAsd19ny9G4rLpMqHDJoZTiU4JmGWtSKbDAZop~QtcDsxnXM0gssf~kEzt6OOsj-OYCtryuomJbh7empuBuMOUR~APpJN1FFZbis79NTy~K7Tyco5xeftY4GK0wJYfudbK0KTQ~ppyrbJEbRuzNn8fJGSLAVghOfW1QPNv4sk47YGwTmJtJBXllu0Kwv7q2JYmQO~X3f7TeluyNFgZz5R~wVgK8~V8Ul2KaAJLQC6~Ak2XBhDyWAVIMnDOgJrKQD4exxVraG4GOFb1PWn8tzpQc84z2DpyhA2QrC5vAWJQ4nicftp9KAXqiMaON0hqX9SsZ9FaZA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of RYR2 activity by luminal Mg2+. The traces were taken from a single experiment, and the luminal [Mg2+] is shown at the left of each trace. The cytoplasmic bath contained 2 mM ATP and 0.1 μM Ca2+ and did not contain any Mg2+. The luminal bath contained 0.1 mM Ca2+. Cytoplasmic and luminal baths also contained 230 mM CsCH3O3S and 20 mM CsCl, pH 7.4. Recordings obtained at +40 mV (top four traces) show channel openings as upward current jumps, whereas recordings at −40 mV show channel openings as downward current jumps.
