Cd²⁺ can form a metal bridge between R294H and A351H. (A) Representative current traces from an oocyte expressing Kv1.2-R294H-A351H channels recorded in the absence (left) and presence (right) of 1 μM Cd²⁺. Black arrowheads indicate where steady-state current was measured to generate I-V and G-V relationships. (B) Mean current–voltage relationships from oocytes expressing Kv1.2-R294H-A351H channels recorded in the absence (solid squares, n = 6) and presence (open circles, n = 6) of 1 μM Cd²⁺. (C) Normalized G-V relationships from oocytes expressing Kv1.2-R294H-A351H channels recorded in the absence (solid squares, n = 6) and presence (open circles, n = 6) of 1 μM Cd²⁺.