Relative effects of proteins containing C1 and the MyBP-C motif on Ca²⁺ sensitivity of tension. Tension–pCa relationships were determined after incubation of trabeculae with 5 μM C1m (upward triangles; n = 6), 5 μM C1C2 (diamonds; n = 4), 5 μM C0C1m (squares; n = 4), and 5 μM C0C2 (downward triangles; n = 6). Data for C1m and C0C1m were redrawn from Fig. 4. Controls curves (circles) were obtained in each trabecula before the addition of recombinant protein.