Schematic diagram of the apparatus used to obtain force measurements and original chart recordings. (Top, inset) Attachment method for securing a permeabilized trabecula to metal troughs. One trough was attached to the active end of a force transducer and the other to a motor for rapid changes in length. (Bottom) Original chart records showing Ca²⁺-activated force in the presence and absence of 5 μM C1C2 or 10 μM C0C1. The pCa values are shown above each activation, and the presence of C1C2 or C0C1 in bath solutions is indicated by bars.