The effect of filtering on the apparent single-channel current. (A and C) No moving average filter (=raw data). (B and D) The same data with moving average filter over 100 data points separated by 5 μs. (A and B) The horizontal lines present I_app as obtained by fit-by-eye (Riessner et al., 2002). (C and D) The vertical lines present I_app as obtained from the maxima of the amplitude histograms of the time series above them. The time series was recorded at −120 mV with 50 mM Tl⁺ + 100 mM K⁺ in the pipette (luminal) and 150 mM K⁺ in the bathing medium (cytosolic).