Figure 1.
Figure 1. Single-channel recordings from outside-out patches. (A) MEC-4d/10d only. (B) MEC-4d/10d + MEC-2. (C) MEC-4d/10d + MEC-6. (D) MEC-4d/10d + MEC-2 + MEC-6. In A–D, two sections are shown: the left demonstrates the sensitivity of the channel to amiloride (50 μM), while the right shows the channel behavior in control saline. Vhold = −150 mV. Closed and open states are labeled C and O respectively and indicated by solid lines. To the right of each record is an all-points histogram of the same trace; dotted lines represent Gaussian fits to each peak. Open probability (E) and single-channel conductance (F) of patches containing a single channel. Data are summarized in Table I. Closed triangles represent the example patches shown in A–D. Bars indicate mean values for each isoform. Number of patches indicated in parentheses. *, significantly different from MEC-4d/10d (P < 0.01).

Single-channel recordings from outside-out patches. (A) MEC-4d/10d only. (B) MEC-4d/10d + MEC-2. (C) MEC-4d/10d + MEC-6. (D) MEC-4d/10d + MEC-2 + MEC-6. In A–D, two sections are shown: the left demonstrates the sensitivity of the channel to amiloride (50 μM), while the right shows the channel behavior in control saline. Vhold = −150 mV. Closed and open states are labeled C and O respectively and indicated by solid lines. To the right of each record is an all-points histogram of the same trace; dotted lines represent Gaussian fits to each peak. Open probability (E) and single-channel conductance (F) of patches containing a single channel. Data are summarized in Table I. Closed triangles represent the example patches shown in A–D. Bars indicate mean values for each isoform. Number of patches indicated in parentheses. *, significantly different from MEC-4d/10d (P < 0.01).

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